Long weekend....

In hopes of finally finishing this current experiment once and for all, I went down to the Biological Station on Friday. I should have known before I ever left that it wasn't going to go well. My professor constantly brings up how I am plagued by bad lusk and this past week was no exception. I was originally planning to leave Wednesday night, but a bad case of food poisoning had me down and out until Friday morning. After getting the car packed (including the kiddo) Friday morning the car wouldn't start. We got it jumped and off we went. On the drive down I called our lab manager to make sure that the water and culture that they had filtered for me was taken out to thaw. It was, but all of the glass jars with water had shattered. Once I got to the station I spent part of the day changing out water baths to get my culture to thaw. In the meantime I tried to get more water filtered. Getting 100% bacteria free anything has been a big challenge for us and the pricy filter we were using was just shot after filtering my culture through, so I had to just go with some pretty dirty water. After 7 hours the culture thawed, but due to baby sitting issues my day had to end. I put everything back in the cold room to start again in the morning. I finally got the experiment set up by about noon the next day, but unfortunetly after a 24 hour assay on fish it was apparent that my culture had lost toxicity after sitting for 24 hours, presumably eaten away by bacteria. Just GRAND! So now I will patiently wait for a month for a new culture to grow and try this all over. I wonder what will go wrong next time?